Table 2.
Experimental groups. Description of experimental groups and animals used in each group.
| Group | Purpose of the group | Number of animals |
|---|---|---|
| Pressure | Optimisation of pressure used to induce ischemia | 20 |
| Control | Determination of baseline enzyme levels and normal histological structure | 6 |
| IR-1 |
Biochemical analysis after 2 h of ischaemia and 10 min of reperfusion |
6 |
| IR-2 |
Biochemical analysis after 2 h of ischaemia and 1 h of reperfusion |
6 |
| IR-3 |
Biochemical analysis after 2 h of ischaemia and 3 h of reperfusion |
6 |
| IR-4 |
Biochemical analysis after 2 h of ischaemia and 6 h of reperfusion |
6 |
| IR-5 |
Biochemical analysis after 3 h of ischaemia and 10 min of reperfusion |
6 |
| IR-6 |
Biochemical analysis after 3 h of ischaemia and 1 h of reperfusion |
6 |
| IR-7 |
Biochemical analysis after 3 h of ischaemia and 3 h of reperfusion |
6 |
| IR-8 |
Biochemical analysis after 3 h of ischaemia and 6 h of reperfusion |
6 |
| IR-9 |
Histopathological analysis after 3 h of ischaemia and 24 h of reperfusion |
6 |
| IR-10 |
Rotarod (functional) analysis after 3 h of ischaemia and 14 days of reperfusion |
6 |
| Total | 86 | |