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. 2021 Sep 29;12(10):890. doi: 10.1038/s41419-021-04153-1

Fig. 2. Construction of cell lines and TNC knockdown attenuated VM formation in vitro.

Fig. 2

A Western blot of TNC protein and gene expression in four gastric cancer cell lines; GAPDH was used as a loading control; protein (B) and gene (C) expression levels of TNC in AGS, BGC-823 and Hs746T cells transfected with siRNA targeting TNC. D Western blot analysis of knockdown efficiency of shTNC. E Bioinformation analysis of TNC functions. F VM was significantly reduced in AGS, BGC-823 and Hs746T cells after transfection with TNC shRNA. Results were shown as mean ± SD of three independent experiments; each experiment was performed in triplicate. **P < 0.01; ***P < 0.001; ****P < 0.0001.