TABLE 1.
Effects of ATPase inhibitors on PPi-driven proton transport in permeabilized procyclic trypomastigotes of T. bruceia
| Inhibitor (concn [μM]) | PPi-dependent H+ transport (ΔA493–530/min × 103) | % of control |
|---|---|---|
| None (control) | 39.2 ± 2.2 | 100 |
| Concanamycin A (0.005) | 34.3 ± 2.2 | 87 |
| Bafilomycin A1 (0.040) | 38.3 ± 3.7 | 97 |
| Sodium o-vanadate (500) | 45.0 ± 1.8 | 114 |
| DCCD (50) | 35.3 ± 0.7 | 90 |
| NBD-Cl (10) | 25.8 ± 2.1 | 65 |
a
Proton transport was assayed by using acridine orange, as described in Materials and Methods, in the standard buffer described in the legend to Fig. 1B. Inhibitors were added after 3 min of permeabilization, prior to the addition of 0.1 mM PPi. The values are means ± SEs of three experiments.