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. 2021 Sep 16;9:741074. doi: 10.3389/fcell.2021.741074

FIGURE 4.

FIGURE 4

Overexpression of miR-29b-3p sensitizes the 3D cultured A549 and MCF7 cells to radiation. (A) Relative expression of miR-29b-3p measured by qRT-PCR in 3D culture miR-29b-3p overexpressed A549 and MCF7 cells. (B) Western blot assay on the expression of c-MYC, DNMT3B, PIK3R1, AKT2, Bcl-2 and RBL1 at indicated time points after 5 Gy X-rays in 3D cultured miR-29b-3p overexpressed A549 cells. (C) The changes of the promoter methylation status of RBL1 measured by MSP in 5 Gy X-ray irradiated and unirradiated 3D cultured miR-29b-3p overexpressed A549 cells. (D) Colony formation assay on the 3D cultured miR-29b-3p overexpressed A549 and MCF7 cells after exposure to 0, 1, 2, 4 and 6 Gy X-rays. Ctrl, unirradiated groups; NC, negative control. Significance was determined by unpaired t-test. *P < 0.05.