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. 2021 Sep 16;9:720349. doi: 10.3389/fcell.2021.720349

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Copyright © 2021 Tao, Liao, Yan, He, Zhou, Wang, Peng, Li and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The depletion of BRCC3 repressed tumor formation in vitro. (A) BRCC3-deficient cells were identified via Western blotting assays. (B) Colony formation assays. (C) An ECIS cell proliferation assay was performed. (D) Three-dimensional colony formation assay was performed in soft agar. The colony numbers are expressed as the means ± standard error of triplicate assays. (E) The Quantitative data show the colonies numbers in (D). (F) Transwell migration assay. The relative cell numbers of migrating were statistically analyzed. (G) Quantitative data show the number of migrating cells in (F). *P< 0.05, **P< 0.001, and ***P< 0.001 compared with controls.