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. 2021 Sep 16;9:720349. doi: 10.3389/fcell.2021.720349

FIGURE 6.

FIGURE 6

The depletion of BRCC3 inhibits bladder cancer tumorigenesis in vivo. (A) Xenograft models (n = 6) were established by subcutaneously inoculating wild-type or BRCC3-KO cells and allowing the cells to grow for 5 weeks. Then the mice were sacrificed and the tumors were removed and weighed. (B–D) The tumor volume and weight measurements are shown. (E) Representative H&E staining and immunohistochemical staining of the xenograft tumors from the tumor-bearing mice in the wild-type and BRCC3-KO groups, showed the deficiency of BRCC3 (the fifth panel) and the upregulation of IκBα expression (the second panel), the downregulation of Ki67 expression (the top panel), TNFα expression (the third panel) and p-P65 expression (the fourth panel) in the BRCC3-KO groups. Statistical analysis of the tumor volume and weight were performed using one-way ANOVA. The means ± standard error of three independent experiments is shown. *P < 0.05, **P < 0.001, and ***P < 0.001 compared with controls.