TABLE 2.
Strains and plasmids used in this study.
| Strain or plasmid | Description | Sources |
|---|---|---|
| Strains | ||
| DH5α | Wild type | Novagen |
| BL21 (DE3) | Wild type | Novagen |
| ML03 | E. coli BL21 (DE3) △cadA | Cheng et al. (2018a) |
| BL21-pET21a | E. coli BL21 (DE3) harboring plasmid pET21a | Cheng et al. (2018b) |
| BL21-raiP | E. coli BL21 (DE3) harboring plasmid pET21a-raiP | Cheng et al. (2018b) |
| BL21-raiP-katE | E. coli BL21 (DE3) harboring plasmid pET21a-raiP-katE | This study |
| ML03-pET21a | E. coli ML03 harboring plasmid pET21a | This study |
| ML03-raiP | E. coli ML03 harboring plasmid pET21a-raiP | Cheng et al. (2018b) |
| ML03-raiP-katE | E. coli ML03 harboring plasmid pET21a-raiP-katE | This study |
| Plasmids | ||
| pET21a-raiP | pET21a carries an L-lysine α-oxidase gene (raiP) from S. japonicus, AmpR | Cheng et al. (2018b) |
| pET21a-raiP-katE | pET21a carries an L-lysine α-oxidase gene (raiP) from S. japonicus and a catalase gene (katE) from E. coli, KanR | This study |