TABLE 3.
Effect of pH on the ratio of 5AVA and δ-valerolactam in ML03-raiP-katE. Data are presented as means ± STDV calculated from three replicate biotransformation experiments. Statistics were performed by the two-tailed Student’s t-test. *p < 0.05; ns, not significant.
| pH | Time (h) | 5AVA production (g/L) | Statistical analysisa | δ-Valerolactam (g/L) | Statistical analysisa | Ratio of 5AVA and δ-valerolactam |
|---|---|---|---|---|---|---|
| 5.0 | 12 | 0.24 ± 0.02 | — | 0.07 ± 0.01 | — | 3.42:1 |
| 24 | 1.12 ± 0.07 | — | 0.25 ± 0.03 | — | 4.48:1 | |
| 6.0 | 12 | 0.28 ± 0.03 | ns | 0.09 ± 0.01 | ns | 3.11:1 |
| 24 | 1.08 ± 0.04 | ns | 0.33 ± 0.03 | ns | 3.27:1 | |
| 7.0 | 12 | 0.31 ± 0.03 | ns | 0.09 ± 0.01 | ns | 3.44:1 |
| 24 | 0.96 ± 0.04 | * | 0.42 ± 0.03 | * | 2.29:1 | |
| 8.0 | 12 | 0.30 ± 0.03 | ns | 0.08 ± 0.01 | ns | 3.75:1 |
| 24 | 0.92 ± 0.05 | ns | 0.56 ± 0.04 | * | 1.64:1 | |
| 9.0 | 12 | 0.27 ± 0.02 | ns | 0.06 ± 0.01 | ns | 4.50:1 |
| 24 | 0.68 ± 0.05 | * | 0.75 ± 0.05 | * | 0.91:1 |
a
Statistical analysis of the 5AVA production was performed with every two separated lines. 6.5 g/L L-lys HCl and 0.2 mM IPTG were added. 10 mM H2O2 was added after 12 h.