TABLE 2.

Isolation of mycoplasmas from urine sediments

Characteristic	% Isolation
Centrifugation speed
500 × g	61.1
2,600 × g	33.9
Growth medium
Broth	51.4
Agar	48.6
Type of growth medium
SP4	37.0
HF	19.2
Friis	19.2
PH	24.6
Other methods
Blind passagea	26.4
Plate washb	8.3
Colony picksc	65.3

^aAt least two blind passages were done with samples which showed no signs of growth before a sample was considered negative for mycoplasmas. 

^bWhen organisms could not be grown from agar blocks, liquid medium was transferred to those plates, and after 24 h of incubation, the liquid medium was aspirated from the agar surface and transferred into broth cultures to obtain stocks. 

^cAgar surfaces showing a colony morphology with a typical fried-egg appearance were cut and transferred into broth cultures.