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Preliminary identification of mycoplasmas isolated from pediatric patients
| Mycoplasma species | Biochemical test result
|
PCR result
|
||||
|---|---|---|---|---|---|---|
| Glucose utiliza-tion | Arginine hydro-lysis | Urease hydro-lysis | Size (bp) of 16S-23S rRNA product | HindIIIb | VspIb | |
| M. penetrans | + | + | − | 295 | − | − |
| M. fermentans | + | + | − | 364 | + | + |
| M. pirum | + | + | − | 323 | + | + |
| M. genitalium | + | − | − | 253 | − | + |
| M. hominis | − | + | − | 236 | − | + |
Second PCR products.
Digestion with restriction enzymes HindIII and VspI.
