| Buffer A | 7 M urea, 20 mM Tris pH 8, add 5 mM β-mercaptoethanol fresh (just before use) |
| Buffer B | 7 M urea, 20 mM Tris pH 8, 1 M NaCl, add 5 mM β-mercaptoethanol fresh |
| Carbonate developing solution | 283 mM sodium carbonate, 0.0185% formaldehyde |
| CutSmart buffer | 50 mM potassium acetate, 20 mM Tris-acetate pH 7.9, 10 mM MgCl2-acetate, 100 μg/mL BSA |
| HAT buffer | 50 mM Tris-HCl pH 8, 10 mM sodium butyrate, 5% glycerol, 0.1 mM EDTA, add 1 mM DTT fresh |
| High salt buffer | 10 mM HEPES pH 7.9, 1.5 mM MgCl2, 1.2 M KCl, 25% glycerol, 0.2 mM EDTA, add 0.2 mM PMSF and 0.5 mM DTT fresh |
| High salt reconstitution buffer | 2 M KCl, 10 mM Tris pH 7.5, 1 mM EDTA, add 1 mM DTT fresh |
| Histone wash buffer | 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM EDTA, add 1mM benzamidine and 5 mM β-mercaptoethanol fresh |
| Hypotonic buffer | 10 mM HEPES pH 7.9, 1.5 mM MgCl2, 10 mM KCl, add 0.2 mM PMSF and 0.5 mM DTT fresh |
| Low salt buffer | 10 mM HEPES pH 7.9, 1.5 mM MgCl2, 20 mM KCl, 25% glycerol, 0.2 mM EDTA, add 0.2 mM PMSF and 0.5 M DTT fresh |
| Low salt reconstitution buffer | 0.2 M KCl, 10 mM Tris pH 7.5, 1 mM EDTA, add 1 mM DTT fresh |
| MOPS SDS running buffer | 50 mM MOPS, 50 mM Tris, 0.1% SDS, 1 mM EDTA |
| NEB3 buffer | 100 mM NaCl, 50 mM Tris-HCl pH 7.9, 10 mM MgCl2, 100 μg/mL BSA |
| Nucleosome loading buffer (8×) | 40% sucrose, 0.1% bromophenol blue |
| Refolding buffer | 10 mM Tris pH 7.5, 2 M NaCl, 1 mM EDTA, add 5 mM β-mercaptoethanol fresh |
| Running buffer | 25 mM Tris, 50 mM glycine, 0.1% SDS |
| TAE buffer | 40 mM Tris, 1 mM EDTA, 20 mM glacial acetic acid |
| TAP buffer | 20 mM HEPES-KOH pH 7.9, 300 mM KCl, 1.5 mM MgCl2, 0.2 mM EDTA, 10% glycerol, add 10 mM sodium butyrate, 10 mM β-glycerophosphate, 1 mM PMSF, 5 mM NaF, 100 μM orthovanadate, 2 μg/mL leupeptin, 2 μg/mL pepstatin, and 5 μg/mL aprotinin fresh |
| TAP wash buffer #1 | 20 mM HEPES-KOH pH 7.9, 300 mM KCl, 0.1% Tween-20, 10% glycerol, add 1 mM DTT, 10 mM sodium butyrate, 10 mM β-glycerophosphate, 1 mM PMSF, 5 mM NaF, 100 μM orthovanadate, 2 μg/mL leupeptin, 2 μg/mL pepstatin, and 5 μg/mL aprotinin fresh |
| TAP wash buffer #2 | 20 mM HEPES-KOH pH 7.9, 150 mM KCl, 0.1% Tween-20, 10% glycerol, add 1 mM DTT, 10 mM sodium butyrate, 10 mM β-glycerophosphate, 1 mM PMSF, 5 mM NaF, 100 μM orthovanadate, 2 μg/mL leupeptin, 2 μg/mL pepstatin, and 5 μg/mL aprotinin fresh |
| TBE (0.2×) | 18 mM Tris, 18 mM boric acid, 0.4 mM EDTA, pH 8.0 |
| Unfolding buffer | 20 mM Tris pH 7.5, 7 M guanidine-HCL, add 10 mM DTT fresh |
| Urea dialysis buffer | 7 M urea, 10 mM Tris pH 8, 1 mM EDTA, 100 mM NaCl, add 5 mM β-mercaptoethanol fresh. |