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. 2021 Sep 16;9:729338. doi: 10.3389/fcell.2021.729338
Buffer A 7 M urea, 20 mM Tris pH 8, add 5 mM β-mercaptoethanol fresh (just before use)
Buffer B 7 M urea, 20 mM Tris pH 8, 1 M NaCl, add 5 mM β-mercaptoethanol fresh
Carbonate developing solution 283 mM sodium carbonate, 0.0185% formaldehyde
CutSmart buffer 50 mM potassium acetate, 20 mM Tris-acetate pH 7.9, 10 mM MgCl2-acetate, 100 μg/mL BSA
HAT buffer 50 mM Tris-HCl pH 8, 10 mM sodium butyrate, 5% glycerol, 0.1 mM EDTA, add 1 mM DTT fresh
High salt buffer 10 mM HEPES pH 7.9, 1.5 mM MgCl2, 1.2 M KCl, 25% glycerol, 0.2 mM EDTA, add 0.2 mM PMSF and 0.5 mM DTT fresh
High salt reconstitution buffer 2 M KCl, 10 mM Tris pH 7.5, 1 mM EDTA, add 1 mM DTT fresh
Histone wash buffer 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM EDTA, add 1mM benzamidine and 5 mM β-mercaptoethanol fresh
Hypotonic buffer 10 mM HEPES pH 7.9, 1.5 mM MgCl2, 10 mM KCl, add 0.2 mM PMSF and 0.5 mM DTT fresh
Low salt buffer 10 mM HEPES pH 7.9, 1.5 mM MgCl2, 20 mM KCl, 25% glycerol, 0.2 mM EDTA, add 0.2 mM PMSF and 0.5 M DTT fresh
Low salt reconstitution buffer 0.2 M KCl, 10 mM Tris pH 7.5, 1 mM EDTA, add 1 mM DTT fresh
MOPS SDS running buffer 50 mM MOPS, 50 mM Tris, 0.1% SDS, 1 mM EDTA
NEB3 buffer 100 mM NaCl, 50 mM Tris-HCl pH 7.9, 10 mM MgCl2, 100 μg/mL BSA
Nucleosome loading buffer (8×) 40% sucrose, 0.1% bromophenol blue
Refolding buffer 10 mM Tris pH 7.5, 2 M NaCl, 1 mM EDTA, add 5 mM β-mercaptoethanol fresh
Running buffer 25 mM Tris, 50 mM glycine, 0.1% SDS
TAE buffer 40 mM Tris, 1 mM EDTA, 20 mM glacial acetic acid
TAP buffer 20 mM HEPES-KOH pH 7.9, 300 mM KCl, 1.5 mM MgCl2, 0.2 mM EDTA, 10% glycerol, add 10 mM sodium butyrate, 10 mM β-glycerophosphate, 1 mM PMSF, 5 mM NaF, 100 μM orthovanadate, 2 μg/mL leupeptin, 2 μg/mL pepstatin, and 5 μg/mL aprotinin fresh
TAP wash buffer #1 20 mM HEPES-KOH pH 7.9, 300 mM KCl, 0.1% Tween-20, 10% glycerol, add 1 mM DTT, 10 mM sodium butyrate, 10 mM β-glycerophosphate, 1 mM PMSF, 5 mM NaF, 100 μM orthovanadate, 2 μg/mL leupeptin, 2 μg/mL pepstatin, and 5 μg/mL aprotinin fresh
TAP wash buffer #2 20 mM HEPES-KOH pH 7.9, 150 mM KCl, 0.1% Tween-20, 10% glycerol, add 1 mM DTT, 10 mM sodium butyrate, 10 mM β-glycerophosphate, 1 mM PMSF, 5 mM NaF, 100 μM orthovanadate, 2 μg/mL leupeptin, 2 μg/mL pepstatin, and 5 μg/mL aprotinin fresh
TBE (0.2×) 18 mM Tris, 18 mM boric acid, 0.4 mM EDTA, pH 8.0
Unfolding buffer 20 mM Tris pH 7.5, 7 M guanidine-HCL, add 10 mM DTT fresh
Urea dialysis buffer 7 M urea, 10 mM Tris pH 8, 1 mM EDTA, 100 mM NaCl, add 5 mM β-mercaptoethanol fresh.