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. 2021 Sep 16;12:698046. doi: 10.3389/fgene.2021.698046

TABLE 1.

Primer sequences used in quantitative reverse-transcription polymerase chain reaction (RT-qPCR)-based verification of RNA sequencing results.

Gene name F-primer R-primer Log2 fold change(DUGL/AGL)
Spp1 TCCACATTTCTGATGACCAGGAT GGGCATGTTCAGACGATGGA 3.76
Col6a1 GCAGTCTTGGAAGGCAATAGG CGAAGGCCAGCCAGAAACAT 0.3
Pla2g6 GTTGGCGCAGCTGATGATG ATGCCCTGGTGAACTTCCAG 1.82
Pla2g4c CTGCAAGTAGTGTAAGGGCT GGGACAAATAAAGACTGCTGGA –2.86
Nr4a3 TTTAACCCATGTCGCTCTGTGA ATCGACTTCAGTGCCTTCGT –3.45
Tfrc TGAAAGTGGAATATCACTTCCTGTC GCTAGGGCCAACTGGTTTCT –1.2
Chrnb1 GTCCTCCTTCAGTGCGTCGT CCTGAATTATCTGCCCCGGAC –1.02
LOC100764246 AAGTCTCTCTCCATATCCTTCCTT GTCCCAGTTAATGCAAAGCCC –2.28
Mpp6 CCACCAAGCTTTTGACGGAC CCCAGCTAATAGGGACCCAC –1.35

DUGL, deep underground lab; AGL, above ground lab.