TABLE 2.

Primer sequences and PCR conditions for genotyping experiments.

Genetic polymorphism	Primer sequence	Tm	Annealing temperature	PCR product size (bp)
FADS1 (rs174556)	Forward inner primer (C allele):	62.5°C	57°C	189 (C allele)
	5′- ACTGACTGTGATTACTATGACTGTGCTC-3′
	Reverse inner primer (T allele):	62.5°C		268 (T allele)
	5′-ATGGGTGGAAGGAGAGTCTAGATTGA-3′
	Forward outer:	62.5°C		404 (two outer primers)
	5′-TGAGCCTGTTATCTCATCTGTAAATGTGT-3′
	Reverse outer:	62.5°C
	5′-GGGCCTTGTGAAGAAGTATATGAACTCT-3′
FADS2 (rs99780)	Forward inner primer (T allele):	65°C	59°C	218 (T allele)
	5′-AATATTAACATGGGAAAAACCGCCCCGT-3′
	Reverse inner primer (C allele):	66°C		297 (C allele)
	5′-AATATATGGAGCGTGGGAACCCGAGATG-3′
	Forward outer:	65°C		460 (two outer primers)
	5′-TAATATAGGTTTGTCTGGAGGCAGGGACTC-3′
	Reverse outer:	64.5°C
	5′-ATATTATAGACGGACCTGTTGCCAAGCC-3′
FADS3 (rs7115739)	Forward inner (T allele):	63°C	61°C	152 (T allele)
	5′-TATACCCGATCTTGGGCCTGATATTAGT-3′
	Reverse inner (G allele):	63.5°C		260 (G allele)
	5′-TATTATTGACATTTCTGGCTCCACCGC-3′
	Forward outer:	64°C		358 (two outer primers)
	5′-TAATGTGGGTTGGGAGAACAGCTGAA-3′
	Reverse outer:	63.5°C
	5′-ATGTACACTTCTCCTTCATGATCTCTCCC-3′