Figure 5.

Predicted breadth and potency of scFv combinations against large virus panel. (A) Scatter plot showing predicted IC₅₀ titers of dual combinations based on single scFv data for a panel of 45 viruses using the Bliss-Hill Independence model. The IC₅₀ data is plotted in μg/mL recalculated from nM based on the formula given in the methods section. (B) Predicted IC₈₀ titers of dual combinations based on the Bliss-Hill Independence model ranked by geometric mean. (C) Breadth-potency curves of IC₅₀ titers of most potent and broad dual combinations were plotted for the IC₅₀.with the others in grey. (D) Breadth-potency curves from IC₈₀ data for the most potent and broad dual combinations. (E) IC₅₀ titers for triple combinations based on the Bliss-Hill Independence model. (F) Predicted IC₈₀ titers for the triple combinations ranked based on geometric mean. (G) Breadth potency curves for the IC₅₀ of the most potent and broad triple combinations are plotted. (H) The breadth-potency curves are based on IC₈₀ titers of the most potent and broad combinations. The maximum neutralization for the IC₅₀ and IC₈₀ data is set at 50μg/mL for all plots. Geometric mean is indicated by a black line in A, C, E, and G Dotted lines are shown at 0.01μg/mL and 1μg/mL for breadth-potency plots IC₅₀ and the IC₈₀ (B, D, F, H). Each scFv is present in the combination at the titer indicated.