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. 2021 Sep 16;12:710067. doi: 10.3389/fmicb.2021.710067

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Copyright © 2021 Li, Baloch, Zhao, Bai, Wang, Wang, Zhang, Lan and Xia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemistry detection in tissues of rKSHV.219-infected tree shrews. (A) The kidney epithelial cells of the TS3 kidney were nuclear staining positive for LANA at 119 dpi. (B) The lymphocytes of the TS4 spleen were nuclear staining positive for LANA at 91 dpi. (C) Hepatocytes of TS3 liver were nuclear staining positive for LANA at 119 dpi. Panels (D–F) were LANA negative control of kidney, spleen, and liver, respectively. (G) The alveolar tissues and lung epithelial cells of TS11 lung were nuclear staining positive for LANA at 14 dpi. (H) The lymphocytes of the TS3 spleen were cytoplasm staining positive for ORF62 at 119 dpi. (I) The alveolar tissues and lung epithelial cells of TS7 lung were cytoplasm staining positive for ORF62 at 56 dpi. (J) was LANA negative control of lung. Panels (K,L) were ORF62 negative control of spleen and lung, respectively. Original magnification, 40×.