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. 2021 Sep 16;9:730925. doi: 10.3389/fbioe.2021.730925

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Copyright © 2021 Zhong, Bejjanki, Miao, Weng, Li, Zhang, Liu, Vannam and Xie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Thermal imaging of the mice after treatment with different formulations with NIR or MHT (A). Imaging was performed before and on day 2 after the different treatments (B). Tissues dissected from nude mice, mass histogram of the tumor weight, mass histogram of the tumor growth inhibition rate, and a line graph of tumor tissue volume over time (C) (N = 5) (*) p < 0.05 (**) p < 0.01 (***) p < 0.001. Immunohistochemical analysis of Hsp70 expression in different groups (D). Figure 4D, i is PBS, ii is PBS + Laser, iii is FA-PEG/CBT@SPION-ICG, iv is FA-PEG@SPION-ICG + MHT, v is FA-PEG/CBT@SPION-ICG + MHT, vi is free ICG + Laser, vii is FA-PEG@SPION-ICG + Laser, and viii is FA-PEG/CBT@SPION-ICG + Laser.