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. 2021 Sep 16;11:712360. doi: 10.3389/fcimb.2021.712360

Figure 5.

Figure 5

Confocal fluorescence microscopy illustrating the distribution and migration of the Staphylococci after 48h colonization. (A) The 3D human epidermal model exhibits differentiated, stratified layers; (B) S. epidermidis remained on the surface of the 3D epidermal model; (C) S. aureus penetrated through the epidermal layers and invaded the bottom layer of the epidermal tissue; (D, E) The presence of S. epidermidis prevented S. aureus from invading the deeper epidermis. SA, S. aureus was inoculated at an infective dose of 103 CFU/well; Mix I, S. epidermidis and S. aureus were inoculated simultaneously at infective dose of 103 and 105 CFU/well, respectively; Mix II, S. epidermidis was inoculated at an infective dose of 105 CFU/well and 4h prior to 105 CFU/well of S. aureus. Bar: 20 μm for the original view and 10 μm for the enlarged view.