Figure 2.

CD11b⁺ macrophages are recruited to the lung during cigarette smoke exposure. Female C57BL/6 mice were RA or CS-exposed for 12-weeks. Lung homogenate (A) immune mediators (CCL2, CCL3, and IL-1α) and (B) monocyte differentiation and macrophage survival factors (M-CSF, GM-CSF, and IL-6). BrdU was used to assess macrophage subpopulation turnover in the lung at 6-weeks CS. Data show (C) total cells, % BrdU⁺ and total BrdU⁺ Res-AM and (D) total cells, total dead and % dead Res-AM. Shown in (E) Mo-AM, (F) IM1, (G) IM2, and (H) IM3 are total cells, % positive and total BrdU⁺ cells. Data show mean ± SEM, n = 4 - 5. Unpaired t test with Welch’s correction. RA, room air; CS, cigarette smoke. *p < 0.05