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. 2021 Sep 16;12:740330. doi: 10.3389/fimmu.2021.740330

Figure 5.

Figure 5

Skewed macrophage subpopulation composition is associated with decreased fibrogenesis. (A) Schematic of CS exposure with bleomycin instillation. C57BL/6 female mice were administered bleomycin (0.05U/mouse) (grey bars) or saline control (open bars) following 12-weeks of RA or CS exposure. Res-AM, Mo-AM, IM1, IM2, and IM3 populations in lung tissue following (B) 7 days or (C) 21 days of bleomycin administration. (D) Functional parameters derived from the pressure-driven pressure–volume loops included: total lung elastance, total lung resistance, lung area (atelectasis) and inspiration capacity measured at day 21. Data show representative image and HALO quantification for (E) α-SMA and (F) Masson’s trichrome. Data show mean ± SEM, n = 5 - 10. Two-way ANOVA with Tukey’s multi-comparison test. Est, elastance; Rrs, resistance; A, area; IC, inspiration capacity; RA, room air; CS, cigarette smoke. *p < 0.05. “-” represents saline administration. "+" represents 0.05U bleomycin administration.