FIGURE 4.

Effect of C12 on yeast response to oxidative stress. A single colony of S. cerevisiae strain BY4741 was inoculated in liquid YPD medium and grown with agitation for 4 h at 30°C. Then, aliquots of this dilution were pre-incubated with 50 μM C12 for 30 min and thereafter exposed to 0.1% H₂O₂ for 10 min. After exposure to oxidative stress the cells were diluted 1:100 in YPD medium and plated on YPD agar. After 96 h, the number of colony forming units (CFU) was counted as a measure of viable cells and compared between treatments. CFU counts for replicate samples are represented by circles (n = 5), and black bars indicate the mean of each group.