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. Author manuscript; available in PMC: 2021 Sep 30.
Published in final edited form as: Biochem J. 2008 Jan 15;409(2):389–398. doi: 10.1042/BJ20070860

Figure 6. Effect of polyamine depletion on the levels of cytoplasmic HuR in stable WT and point-mutated Impα1-transfected IEC-6 cells.

Figure 6

Whole cell lysates were harvested after control (Con) IEC-6 cells (left), stable WT-Impα1-transfected cells (middle) and stable point-mutated Impα1-transfected cells (right) were grown in the control cultures and cultures containing DFMO (5 mM) alone or DFMO plus putrescine (10 μM) for 6 days. Total and cytoplasmic proteins were prepared and levels of AMPKα and HuR were measured by Western-blot analysis. Immunoblottings of β-actin in total cell lysates and β-tubulin in cytoplasmic lysates were examined to serve as equal loading controls. Three experiments were performed and they showed similar results.