Table 1.
Generation of single-color controls for sorting of mouse LT-HSC
| Tube | HBSS++ | Biotin-labeled lineage antibodies | PE-Cy7 (Sca1) | APC (c-kit) | Pacific blue (CD150) | APC-Cy7 (CD48) | PE-streptavidin |
|---|---|---|---|---|---|---|---|
| Unstained (autofluorescence) | 100 μL | - | - | - | - | - | - |
| PE single-color control |
100 μL | 2.5 μL of each | - | - | - | - | 1 μL |
| PE-Cy7 single-color control |
100 μL | - | 2.5 μL | - | - | - | - |
| APC single-color control |
100 μL | - | - | 2.5 μL | - | - | - |
| Pacific Blue single-color control |
100 μL | - | - | - | 2.5 μL | - | - |
| APC-Cy7 single-color control |
100 μL | - | - | - | - | 2.5 μL | - |
| Experimental sample 1 | 1000 μL | 25 μL of each | 25 μL | 25 μL | 25 μL | 25 μL | 7 μL |
| Experimental sample 2 | 1000 μL | 25 μL of each | 25 μL | 25 μL | 25 μL | 25 μL | 7 μL |
| Experimental sample n | 1000 μL | 25 μL of each | 25 μL | 25 μL | 25 μL | 25 μL | 7 μL |