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Column buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| EDTA (0.5 M) | 2 mM | 2 mL |
| BSA | 0.5% | 2.5 g |
| PBS | 1× | 500 mL |
| Total | n/a | 502 mL |
Stir on a magnetic stir plate until completely dissolved and then filter through a 0.45 μm filter. Keep sterile.
Store at 4°C; maximum storage 1 month.
