FIGURE 2.

(A) and (B) Effect of warifteine (WAR) and TAFrz subfractions on virus titer. Vero cells were infected with a Multiplicity of Infection (MOI) of 0.1 of ZIKV for 2 h. The cell monolayer was then treated with the corresponding CC₂₀ of each sample and incubated for 120h, and the supernatant collected and titrated using the TCID₅₀ method. Values of concentration are given in µg/mL. Results are expressed as mean ± SD of 3 independent experiments. *p < 0.05 in relation to untreated Vero Cells (DMEM group). (C) Protection of Vero Cells from ZIKV-induced cytopathic effect by WAR and 5-MMPr. Mock (negative control) corresponds to non-infected cells treated only with DMEM. ZIKV (positive control) corresponds to cells infected with ZIKV and not treated.