Table 2.
PCR oligonucleotide primers for transconjugants genotyping.
| Name | Sequence (5′ to 3′) | Target (Bacterial Species) | Direction |
|---|---|---|---|
| IF327 IF328 |
CAATATAGCGTGATGATTGTAAT AGTGAGAATCAAATCAGAGGTT |
5’ end of Tn5253, Tn5253-like 3’ end of Tn5253, Tn5253-like |
Reverse Forward |
| IF325 IF356 |
ACAAGAACTGTTTGGACATCAT GACTAGATAGAGGCAAGCGT |
Tn5253, Tn5253-like chromosomal integration site (S. pneumoniae) | Forward Reverse |
| IF560 IF561 |
AACGAAACCTATCAGCGGAA TTTGGGTTTGTCTCCGACGA |
Tn5253, Tn5253-like chromosomal integration site (S. agalactiae) | Forward Reverse |
| IF512 IF513 |
TGCTTTAGGAGATGTTGAGTT ACCGCAGACTGTTCTTTAGA |
Tn5253, Tn5253-like chromosomal integration site (S. gordonii) | Forward Reverse |
| IF509 IF510 |
AAGTAGAAATGGCGAAGTGAA GACTAGAAAGTGGTAAGCGT |
Tn5253, Tn5253-like chromosomal integration site (S. pyogenes) | Forward Reverse |
| IF532 IF525 |
GCCTATGGGATTGCTACACC GGTTACGGGAAGAAAGCGGT |
Tn5253, Tn5253-like chromosomal integration site (E. faecalis) | Forward Reverse |
| IF855 IF327 |
ACCAAATTCCTGCCAGAGTTGA CAATATAGCGTGATGATTGTAAT |
5’ end of ICESp23FST81 3’ end of ICESp23FST81 |
Reverse Forward |
| IF345 IF347 |
ATGGTAATCATCTAAAAATGTCAC CACCAGCACTTGTTAAAGAAG |
ICESp23FST81chromosomal integration site (S. pneumoniae) |
Forward Reverse |
| IF394 IF564 |
GCTATAGTATAAGCCATACTT GAAGTGACTTGTGCTCTGCT |
Tn5253-family tet (M) resistance gene | Forward Reverse |
| IF354 IF353 |
CATTCTCTGGTATTTGGACTC CTCTCCGTCGCTATTGTAAC |
Tn5253-family cat resistance gene | Forward Reverse |
| IF127 IF162 |
TGTTCTTCATCTACTACGACTG TGATGATTATATAAATTGTGAGTT |
5’ end of Φ1207.3 3’ end of Φ1207.3 |
Reverse Forward |
| IF281 IF282 |
AGGTGGTAAGGCAGAATC GCACCTTTGTTTGAGTCG |
Φ1207.3 chromosomal integration site (S. pneumoniae) |
Forward Reverse |
| IF302 IF303 |
AATAGATGTAGGTGGGCG AGCTTTGGCAACCACTTC |
Φ1207.3 chromosomal integration site (S. pyogenes) |
Forward Reverse |