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RNA-sequencing workflow. RNA-seq is a three-step method: (1) library construction; (2) sequencing; (3) bioinformatic analysis. The RNA species of interest is selected and converted to complementary DNA, which is then amplified by PCR in order to prepare a sequencing library. Sequencing results in the generation of short sequences (reads) that need to be aligned to a reference genome. Then, different approaches can be used for transcript assembly to detect quantitative gene expression.
