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Yth1 is not detected in Cft1 immunoprecipitates of CF II. Cell extract (lanes 2 to 4) or a CF II phosphocellulose fraction (lane 5) was immunoprecipitated with the indicated antibodies, and blots of the immunoprecipitates were probed with antibody against Cft1, Pap1, or Yth1. Recombinant Yth1 produced in E. coli is shown in lane 1, and an immunoprecipitation with preimmune serum is shown in lane 2.
