FIG. 4.

Immunoblot analysis of cell extract from wild-type and pta1 mutant strains. Equal amounts of protein (15 μg) from wild-type (lanes 1 and 2), pta1-1 (lane 3), or pta1-2 (lane 4) extracts were separated on an SDS–8% polyacrylamide gel, transferred to a polyvinylidene difluoride membrane, and immunoblotted with a monoclonal antibody against Pta1 (A), Cft1/Yhh1 (B), Brr5/Ysh1 (C), or Pap1 (D). Lane 1 is a control blot probed with preimmune antiserum from a mouse subsequently immunized with Pap1 antigen.