FIG. 5.

Full-length Pta1 protein is found in a Pap1-containing complex. An equal amount of extract from wild-type (WT) or pta1-1 mutant cells was subjected to immunoprecipitation with monoclonal antibody against Pap1. The immunoprecipitated proteins were fractionated on an SDS–8% polyacrylamide gel, transferred to a polyvinylidene difluoride membrane, and immunostained with antibody against Pta1 (A), Cft1/Yhh1 (B), Brr5/Ysh1 (C), or Pap1 (D). Lane 1, wild-type extract; lane 2, pta1-1 mutant extract; lane 3, wild-type extract immunoprecipitated with anti-immunoglobulin G (IgG) antibody.