FIG. 7.
Complementation of polyadenylation activity in the pta1-1 mutant extract. Reactions were performed as described in the legend to Fig. 6, and reaction mixtures were incubated at 30°C for 30 min. 32P-labeled GAL7-1 RNA was incubated with extracts of wild-type (WT) (lane 2), pta1-1 (lane 3), brr5-1 (lane 4), rna14-1 (lane 5), and fip1-1 (lane 6) strains. Lanes 7 to 9 are reaction mixtures containing pta1-1 extracts combined with other mutant extracts as indicated. Lanes 10 to 14 are reaction mixtures containing different mutant extracts or buffer as indicated and supplemented with CF II. The reaction mixture of rna14-1 extract in the presence of a CF I fraction (from the heparin-Sepharose step) is shown in lane 15. See the Fig. 6 legend for symbol definitions.