FIG. 8.
Pta1 is required for both cleavage and poly(A) addition of CYC1 pre-mRNA substrate. Reactions were carried out as described in the legend to Fig. 6. (A) Coupled cleavage-polyadenylation assays in the presence of ATP. (B) Cleavage assays with 2′-dATP. 32P-labeled CYC1 substrates were combined with extracts of wild-type (WT) (lane 2), pta1-1 (lane 3), and pta1-2 (lane 4) strains and pta1-1 and pta1-2 strains plus CF II (lanes 5 and 6, respectively). In lane 6, a reaction was performed with CF II only. See the Fig. 6 legend for symbol definitions.