Sequences of the oligonucleotides^a.

Oligonucleotides	Sequence (5′–3′)
miR-155	UUA AUG CUA AUC GUG AUA GGG GU
miR-21	UAG CUU AUC AGA CUG AUG UUG A
Linear template-155	TCA CTA ACT GGC TCT GTA CGA CTC AAA TTT ACC CCT ATC ACG ATT AGC ATT AA – NH2
Linear template-21	AGT GTA TGT CCT GGA AGA CGA CTC AAA TTT TCA ACA TCA GTC TGA TAA GCT A – NH2
AP probe-155	Biotin – AAC ATC CCT AAT TTC TCA CTA X CTG GCT CT – NH2
AP probe-21	Biotin – AAG ATG GGT AAT TAG AGT GTA X GTC CTG GA – NH2
Circular template-155	TAG TGA GAA ATT AGG GAT GTT AAG TAG GAT GTT GAG TAA AGT TGA AGA ATG GTG A
Circular template-21	TAC ACT CTA ATT ACC CAT CTT AAC TAC CAT CTT CAC TAT TCA ACT TCA ATC CTC A
Trigger-155	AGA GCC AGT TAG TGA
Trigger-21	TCC AGG ACA TAC ACT
let-7a	UGA GGU AGU AGG UUG UAU AGU U
let-7b	UGA GGU AGU AGG UUG UGU GGU U
let-7c	UGA GGU AGU AGG UUG UAU GGU U
mis-1-155	TTA ATG CTA ATC GTG ATA GGG C̲T
mis-2-155	TTA ATG CTA ATC GTG ATA C̲GG C̲T
mis-3-155	TTA ATG CTA ATC GTG T̲TA C̲GG C̲T
mis-1-21	TAG CTT ATC AGA CTG ATG TTC̲ A
mis-2-21	TAG CTT ATC AGA CTG ATC̲ TTC̲ A
mis-3-21	TAG CTT ATC AGA CTC̲ ATC̲ TTC̲ A

^aIn the linear templates, the recognition sites of Nt.BstNBI are shown in bold. In the AP probes, the letter “X” represents an AP site. In the mismatched RNAs, the underlined bases indicate the mismatched bases.