. Author manuscript; available in PMC: 2023 Sep 6.

Published in final edited form as: Anal Chem. 2021 Sep 20;93(38):13063–13071. doi: 10.1021/acs.analchem.1c03016

Table 1:

Sensitivity of direct RT-LAMP, RT-RPA, and Closed-Tube Penn-RAMP for the detection of SARS-CoV-2 eluted from nasal swabs (contrived samples)^*.

Swab elution media	Spiked samples	RT-LAMP^**	RT-RPA^**	Penn-RAMP^**
VTM	10000 particles/reaction	4/4	4/4	4/4
	1000 particles/reaction	4/4	4/4	4/4
	100 particles/reaction	2/4	1/4	4/4
	10 particles/reaction	0/4	0/4	0/4
	0 particles/reaction	0/4	0/4	0/4

Water	10000 particles/reaction	4/4	4/4	4/4
	1000 particles/reaction	4/4	4/4	4/4
	100 particles/reaction	4/4	4/4	4/4
	10 particles/reaction	0/4	0/4	3/4
	0 particles/reaction	0/4	0/4	0/4

We submerged a nasal swab in 3 mL of VTM and water and spike SARS-CoV-2 into each of them to prepare contrived nasal swab samples.

^**

The table documents the fraction of positive results.