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. 2021 Sep 16;6(38):24834–24847. doi: 10.1021/acsomega.1c03681

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© 2021 The Authors. Published by American Chemical Society

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Validation of selected binding partners of FOSL1 and FOSL2 (A,B) FOSL1 (panel A) and FOSL2 (panel B) protein was immunoprecipitated from 72 h-cultured Th17 cells and western blotting was used to confirm their MS-identified interactions with SIRT-1, JUNB, RUNX1, and JUN. Blots depict lanes for total lysate (input), control IgG IP, and FOSL1/FOSL2 IP. Figures show representative blots for two or three biological replicates (see Figure S5 for all the replicates). (C,D) Volcano plots show selected binding partners of FOSL1 (panel C) and FOSL2 (panel D) that were validated by PRM-MS analysis. Data are representative of three biological replicates. The plots were extracted from Skyline.