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A-443654 effects on the AKT pathway. Western blot of phospho- and total AKT and phospho-GSKα/β in HEK-293 cells treated with increasing doses of A-443654 (left). Quantification of three independent experiments (right). ∗p < 0.05 and ∗∗p < 0.01, Student’s t test with Bonferroni correction.