Figure 6.

Effects of inactivated whole virion influenza vaccine containing alum

Mice were immunized with WV alone and WV plus alum subcutaneously.

(A) Levels of WV-specific total mIgG, mIgG1, mIgG2b, and mIgG2c in the plasma were determined after final immunization. We used 4,000- (●), 20,000- (◆), and 100,000- (▲) fold–diluted plasma samples.

(B) Neutralization titers against Cal7 and PR8 in the plasma samples obtained from immunized mice were determined using MDCK cells. Plasma samples obtained from PR8-immunized mice were used as a positive control (PC). N.D.: not detected. The dashed line shows the detection threshold for a positive response.

(C) Splenocytes obtained from immunized mice were incubated in the presence of WV in vitro and the levels of IFN-γ in the supernatants were measured after 3 days. (A–C) n = 5 per group. Data are means ± SD. (A and C) ∗∗p<0.01, ∗∗∗∗p<0.0001 as indicated by Tukey’s test. (A) Significant differences were analyzed only in the 4,000-fold-diluted plasma samples. (B) ∗∗p<0.01 as indicated by Student’s t-test.