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. 2021 Sep 30;16:69. doi: 10.1186/s13024-021-00482-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Cross-species comparison of MC gliosis in response to laser injury. (A-E) Analysis of MC gliotic response in zebrafish and mice at baseline (Uninjured) and different time point after injury (Day 1, 3, 7 and 14). Detection of GFAP in GS⁺MCs after laser induction in zebrafish (A.i-A.v) and mice (B.i-B.v). Shown are sections for GS (red) and GFAP (green). Detection of phospho-Erk1/2 in GS⁺MCs after injury and in uninjured zebrafish (C.i-C.v) and mice (D.i-D.v). Shown are sections for GS (red) and phospho-Erk1/2 (green). Histograms illustrating mean ± SD of GS⁺, GFAP⁺ and pERK1/2⁺cells normalized by the total of DAPI⁺ or GS⁺ cells in percentage (G). Significant differences (***p < 0.001, ****p < 0.0001) between uninjured and injured retinas were determined by post-hoc Bonferroni one-way ANOVA test (n = 12). (E-F; H) Evaluation of the proliferative potential of zebrafish and murine MCs during injury response at baseline (Uninjured) and different time point after injury (Day 1, 3, 7 and 14). Detection of PCNA in GS⁺MCs after injury in zebrafish (E.i-E.v) and mice (F.i-F.v). Shown are sections for GS (red) and PCNA (green). Histograms illustrating mean ± SD of PCNA⁺cells normalized by the total of GS⁺cells in percentage (H). Significant differences (****p < 0.0001) between uninjured and injured retinas were determined by post-hoc Bonferroni one-way ANOVA test (n = 12)