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. 2021 Sep 30;16:69. doi: 10.1186/s13024-021-00482-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Analysis of cellular DNA content. (A) Flow cytometry of cellular DNA content (Hoechst) of zebrafish gfap:gfap-GFP⁺ and murine Rlbp1:GFP⁺ MCs. (B) Representative histograms illustrating mean ± SD of Hoechst⁺ cells in the different phases of the cell cycle normalized by the total of GFP⁺MCs in percentage at different time points (Day 1, 3, 5 and 7). Significant differences (*p < 0.05, **p < 0.01 and ***p < 0.001) between uninjured and injured at the different time points were determined by post-hoc Bonferroni one-way ANOVA test (n = 12). INL, inner nuclear layer; ONL, outer nuclear layer. Scale bar of the images equals 50 μm