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. 2021 Sep 30;16:69. doi: 10.1186/s13024-021-00482-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Pharmacological Notch inhibition (DAPT) increases N-cadherin expression. Mice were treated with DAPT (8 mg/kg) by intraperitoneal injection either at 3 h before injury, at day 2 or at day 6 (syringes) and euthanized 24 h after injection (orange arrows; Day 1, 3, 7, respectively). (A-B) Analysis of Notch isoforms during MC injury response in DAPT treated mice at different time points (Day 1, 3 and 7). Detection of Notch1 (A.i-A.iii) and Notch2 (B.i-B.iii) in GS⁺ MCs. Shown are representative sections stained for GS (red), Notch1/2 (green). (C-D) Analysis of MC phenotype during injury response in DAPT treated mice at different time points (Day 1, 3 and 7). Detection of E-cadherin (C.i-C.iii) and N-cadherin (D.i-D.iii) in GS+ MCs. Shown are representative sections stained for GS (red), E- and N-cadherin (green). (C.iv, D.iv) Histograms illustrating the mean ± SD of the number of Notch1⁺ and Notch2⁺ cells normalized by the total number of GS⁺ cells expressed in percentage (n = 12)