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. 2021 Sep 30;20:191. doi: 10.1186/s12934-021-01672-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Impact of harvest time in fed-batch GFP1-10 production on quality of GFP1-10 detector solution. Samples for preparation of GFP1-10 detector solution were taken 8, 9, 10 and 11 h after induction of GFP1-10 expression in E. coli BL21(DE3) pET22b-sfGFP1-10 with IPTG. A Cell dry weight of fed-batch fermentation in DeLisa defined medium. Dashed black lines indicate start and end of feed-phase and dotted lines indicate sampling times. B Split GFP assay with detector solutions derived from fermentation samples 8, 9, 10 and 11 h after induction. Supernatant containing cutinase-GFP11 was mixed with the detector solutions, respectively. Data is shown as mean of eight technical replicates with standard deviation