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. 2021 Sep 30;20:191. doi: 10.1186/s12934-021-01672-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Application of split GFP assay in screening of cutinase-GFP11 secretion. C. glutamicum pPBEx2-NprE-Cutinase-GFP11 and pPBEx2-Pel-Cutinase-GFP11 growth curves by backscattered light (A) and dissolved oxygen (B). Confidence tubes deviated from 24 biological replicates per strain. Each replicate was induced with IPTG individually triggered by backscatter signal. The period in which all samples were induced is highlighted in gray. Cells were harvested 4 h after induction and cutinase-GFP11 in supernatant was detected by split GFP and cutinase activity assay (C). Error bars deviated from 24 biological and two technical replicates. Fluorescence signal of split GFP assay was measured after 16 h incubation at 20 $^{\circ}$ C