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. 2021 Sep 30;21:1069. doi: 10.1186/s12885-021-08668-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — MCF2L-AS1 is highly expressed in CRC cells and accelerates the progression of CRC. A. The expression of MCF2L-AS1 in COAD tissues and adjacent normal tissues was assessed via GEPIA database. B. MCF2L-AS1 expression was measured in CRC cell lines (HCT15, SW620, SW116 and LOVO) and human normal colon epithelial cells (NCM-460) by RT-qPCR. C. The interference efficiency of MCF2L-AS1 in HCT15 and SW116 cells was tested by RT-qPCR assay. D and E. Cell proliferation in HCT15 and SW116 cells after MCF2L-AS1 silencing was evaluated through IF staining and EdU assay. F and G. The migratory and invasive abilities of CRC cells upon MCF2L-AS1 silencing were evaluated through Transwell assays. H and I. Flow cytometry assay and caspase-3/8/9 activity analysis were utilized to test cell apoptosis upon MCF2L-AS1 silencing. J. The EMT process was assessed through IF assay after MCF2L-AS1 was silenced in HCT15 and SW116 cells. Adjustments of individual color channels were made on ‘Merge’ figures. The statistical analysis of Fig. 1B-I was tested with one-way ANOVA. *P < 0.05, **P < 0.01