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. Author manuscript; available in PMC: 2021 Sep 30.
Published in final edited form as: Nat Struct Mol Biol. 2020 Feb 10;27(2):202–209. doi: 10.1038/s41594-020-0373-0

Extended Data Fig. 1 |. Biochemical characterizations of recombinant mBcs1.

Extended Data Fig. 1 |

a, Size exclusion chromatographic profile of mBcs1 on Superdex 200. Inset: SDS-PAGE showing the purity of mBcs1 along the purification steps. b, One representative Michaelis Menten plot out of three experiments of mBcs1 ATPase activity to ATP concentration (n=3 technical replicates). c, Nucleotide-dependent conformational change of mBcs1 in the presence of different nucleotides as observed by Blue-Native PAGE.