FIG. 3.

Determination of the relative levels of Sis1 and Ydj1 in wild-type cells. Wild-type cells (PJ43-2B) were grown at 30°C in YEPD media to an OD₆₀₀ of 0.5. Approximately 0.3 OD₆₀₀ U of cells was collected, and 200-μl cell lysates were made from these cells. Ten microliters of these cell lysates and the indicated amounts of purified Sis1 and Ydj1 were run on SDS-PAGE and immunoblotted with anti-Sis1 and anti-Ydj1 antibodies. Immunoblot signals from cell lysates were compared with those from the predetermined amounts of Sis1 or Ydj1. The cell lysate was calculated to contain about 19 fmol of Sis1 and 285 fmol of Ydj1.