FIG. 4.

Amino acid changes in the HPD loop disrupt Sis1 function. Diagram of sis1 point mutations H34Q (single star) and HPD34-36AAA (triple stars) in truncated (amino acids 1 to 121) or full-length Sis1 (WT). WY26 cells containing these mutant constructs were diluted and spotted on 5-FOA plates; the plates were incubated at 30°C for 2 days (truncation constructs) and 3 days (full-length constructs), respectively.