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. 2021 Aug 18;3(1):vdab104. doi: 10.1093/noajnl/vdab104

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© The Author(s) 2021. Published by Oxford University Press, the Society for Neuro-Oncology and the European Association of Neuro-Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — n-siRNA uptake and functional activity in rat C6 glioma cells. (A) Representative confocal images showing n-Cy5-siRNA uptake in (i) C6 cells (red) (ii) corresponding merged image (iii) merged confocal image in C6 neurospheres. (B) Flow analysis of n-siRNA uptake showing (i) unstained, (ii) naked-siRNA, and (iii) n-siRNA (Alexaflour 555) in C6 neurospheres, indicating uptake in 98.7% cells. (C) Relative gene expression levels of FAK, NOTCH-1, and SOX-2 gene analyzed by quantitative real-time PCR after treatment with n-siRNA_FAK, n-siRNA_NOTCH-1 or siRNA_SOX-2 (100 nM each), *P < .05 compared to corresponding untreated. (D) Wound healing assay showing inhibition of cell migration in n-siRNA_FAK treated C6 glioma cells. Data are mean ± standard deviation.