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. 2021 Sep 16;14:727552. doi: 10.3389/fnmol.2021.727552

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Copyright © 2021 Mou, Dein, Chen, Jagdale and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of motor neurons in Luc RNAi, MFN2 RNAi #1, and MFN2 RNAi #2. (A) Immunostaining of spinal motor neuron progenitor markers, OLIG2 (magenta) in D28-old neurons. (B) Quantification showing the population of OLIG2⁺ in D28 neural cultures. (C) Immunostaining of spinal motor neuron marker, HB9 (blue) and axonal marker TAU (red) in D35-old neurons. Scale bar: 20 μm. (D) Quantification of HB9⁺ spinal motor neurons in Luc RNAi, MFN2 RNAi #1, and MFN2 RNAi #2 at D35 of neural culture. Data are represented as mean ± SEM, n = 3 independent experiments.