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. 2021 Sep 29;8(5):ENEURO.0276-21.2021. doi: 10.1523/ENEURO.0276-21.2021

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Copyright © 2021 Antonellis et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 1. — Hedgehog pathway expression in the adult mouse ARC. Dual-probe in situ hybridization labeling of the ARC with probes to hedgehog pathway and neuronal gene transcripts. A–J, Pomc (A–E) and Agrp (F–J) probes are labeled in red, while Shh (A, F), Ptch1 (B, G), Smo (C, H), Gli1 (D, I), and Gpr161 (E, J) probes are labeled in blue. Examples of cells colabeled by both probes are denoted by an asterisk (*). Pomc- or Agrp-expressing cells adjacent to highly expressing Ptch1 (B, G) or Gli1 (D, I) cells are denoted by an arrow. Right-hand panels are magnified images of the region shown in a black box on the left-hand side. Scale bars: left panels, 100 μm; right panels, 50 μm. V3, Third ventricle.