Figure 4.

Gli1 and Ptch1 expression in astrocytes in adult mouse hypothalamus. Dual-probe in situ hybridization was performed with probes for Gli1 or Ptch1 and astrocyte marker Gfap gene transcripts. A–D, Sections of the ARC (A, C) and PVN (B, D) from adult mice were colabeled with probes to Gli1 or Ptch1 labeled in blue and probes to Gfap labeled in red. Example cells that are highly positive for both probes are denoted by an arrow. Right-hand panels are magnified images of a region shown in a black box on the left-hand side. Scale bars: left panels, 100 μm; right panels, 50 μm. V3, Third ventricle.