Table 2.
Cloning primersa
| Primers | 5′-3′ sequence |
|---|---|
| mSLK-GFP-fw | GCGCCCGGGATGTCCTTCTTCAATTTCCGTAAG |
| mSLK-GFP-rev | GCGACCGGTCCTGACCCAGTGGAATGTAAG |
| mSLK-mCherry-fw | GCGCCCGGGATGTCCTTCTTCAATTTCCGTAAG |
| mSLK-mCherry-rev | GCGACCGGTCCTGACCCAGTGGAATGTAAG |
| hSLK-GFP-fw | GCGGAATTCACCATGTCCTTCTTCAATTTCCGTAAGA |
| hSLK-GFP-rev | ACCGGTTGATCCGGTGGAATGCAAGC |
| hSLK-mCherry-fw | GCGGAATTCACCATGTCCTTCTTCAATTTCCGTAAGA |
| hSLK-mCherry-rev | ACCGGTTGATCCGGTGGAATGCAAGC |
| shRNA against SLK for pAAV plasmids: shSLK-fw | GATCTCGGGTTGAGATTGACATATTAATAGTGAAGCCACAGATGTATTAATATGTCAATCTCAACCTTTTGGAAA |
| shSLK for pAAV plasmids: shSLK-rev | AGCTTTTCCAAAAGGTTGAGATTGACATATTAATACATCTGTGGCTTCACTATTAATATGTCAATCTCAACCCGA |
| shSLK for pLVTHM plasmids: shSLK-fw | CGCGTGGTTGAGATTGACATATTACTCGAGTAATAT GTCAATCTCAACCTTTTTTAT |
| shSLK for pLVTHM plasmids: shSLK-rev | CGATAAAAAAGGTTGAGATTGACATATTACTCGAGTAATATGTCAATCTCAACCA |
aThe primer pairs were designed to clone constructs used in this study. fw, forward primer; rev, reverse primer.