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. 2021 Sep 29;41(39):8111–8125. doi: 10.1523/JNEUROSCI.0352-21.2021

Table 2.

Cloning primersa

Primers 5′-3′ sequence
mSLK-GFP-fw GCGCCCGGGATGTCCTTCTTCAATTTCCGTAAG
mSLK-GFP-rev GCGACCGGTCCTGACCCAGTGGAATGTAAG
mSLK-mCherry-fw GCGCCCGGGATGTCCTTCTTCAATTTCCGTAAG
mSLK-mCherry-rev GCGACCGGTCCTGACCCAGTGGAATGTAAG
hSLK-GFP-fw GCGGAATTCACCATGTCCTTCTTCAATTTCCGTAAGA
hSLK-GFP-rev ACCGGTTGATCCGGTGGAATGCAAGC
hSLK-mCherry-fw GCGGAATTCACCATGTCCTTCTTCAATTTCCGTAAGA
hSLK-mCherry-rev ACCGGTTGATCCGGTGGAATGCAAGC
shRNA against SLK for pAAV plasmids: shSLK-fw GATCTCGGGTTGAGATTGACATATTAATAGTGAAGCCACAGATGTATTAATATGTCAATCTCAACCTTTTGGAAA
shSLK for pAAV plasmids: shSLK-rev AGCTTTTCCAAAAGGTTGAGATTGACATATTAATACATCTGTGGCTTCACTATTAATATGTCAATCTCAACCCGA
shSLK for pLVTHM plasmids: shSLK-fw CGCGTGGTTGAGATTGACATATTACTCGAGTAATAT GTCAATCTCAACCTTTTTTAT
shSLK for pLVTHM plasmids: shSLK-rev CGATAAAAAAGGTTGAGATTGACATATTACTCGAGTAATATGTCAATCTCAACCA

aThe primer pairs were designed to clone constructs used in this study. fw, forward primer; rev, reverse primer.